技术原理
单克隆抗体是由单一B细胞克隆产生的高度均一、仅针对某一特定抗原表位的抗体。它在药物研发、诊断试剂开发中扮演了关键的角色。抗体分子本的一级结构，特别是它的CDR区域氨基酸序列是其发挥生物学功能的核心。因此，对单抗分子进行准确快速的鉴定分析具有重要的意义。通过先进的高分辨率质谱仪Obitrap Fusion Lumos，结合丰富的生物信息学分析经验，可以对不同亚型抗体（例如IgG和IgM）和不同类型抗体（荧光偶联抗体，固定化抗体，不同物种的抗体）的一级序列信息进行快速准确的分析。
 
 

单克隆抗体测序技术原理图

技术特点
l  不基于核酸测序与杂交瘤细胞；
l  获得抗体的轻重链完整氨基酸，可以直接表达；
l  能够区分I/L;
l  周期快（20天，加急2周出结果）
 
样品要求
l  纯度>95%(SDS-PAGE胶图，HPLC或者是质谱分子量证据，可提供纯度更低的样品解析服务，具体电联）；
l  只需要少至100ug样品（可提供更低样品量的解析服务，具体电联）；
 
费用返还保证

注意事项
l  请使用足量的干冰运输，并尽量选用较快的邮递方式，以降低运输过程中样品降解的可能性。
 
项目报告
l  实验步骤
l  质谱参数
l  质谱谱图
l  单抗分子序列的详细信息
 
单克隆抗体测序一站式服务
l  您只需下单-寄送样品
l  明德正康为您完成-样品处理-上机分析-数据分析-项目报告

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